Uji Aktivitas Antioksidan Ekstrak Etanol Daun Pepaya (Carica papaya L.) dengan Metode DPPH
DOI:
https://doi.org/10.55606/jikki.v5i2.6231Keywords:
Papaya leaf, Antioxidant, DPPH, QuercetinAbstract
Oxidative stress is a condition resulting from an imbalance between free radicals and the body's antioxidant system, which can damage cells and trigger degenerative diseases such as diabetes, cancer, atherosclerosis and heart disease. Antioxidants play an important role in neutralizing free radicals to prevent cell damage. Papaya leaves (Carica papaya L.) are known to contain bioactive compounds that have potential as a source of natural antioxidants. This study aims to test the antioxidant activity of papaya leaf ethanol extract using the DPPH (2,2-diphenyl-1-picrylhydrazyl) method, with quercetin as a comparator. Extraction was done by maceration using 80% ethanol. Antioxidant activity was measured based on the decrease in absorbance of DPPH solution at a wavelength of 517 nm using UV-Vis spectrophotometer. The results showed that papaya leaf extract had strong antioxidant activity with IC₅₀ value of 21.35 ppm, while quercetin was 4.84 ppm. Thus, the ethanol extract of papaya leaves showed high potential as an effective natural antioxidant.
References
Bulla, R. M., Da Cunha, T. M., & Nitbani, F. O. (2020). Identifikasi dan uji aktivitas antioksidan senyawa alkaloid daun pepaya (Carica papaya L.) kultivar lokal. Chemistry Notes, 2(1), 58–68.
Dalimunthe, A., Pertiwi, D., Muhmmad, M., Kaban, V. E., Nasri, N., & Satria, D. (2022). The effect of extraction methods towards antioxidant activity of ethanol extract of Picria fel-terrae Lour. herbs. IOP Conference Series: Earth and Environmental Science, 1115(1), 012040.
Di Meo, S., & Venditti, P. (2020). Evolution of the knowledge of free radicals and other oxidants. Oxidative Medicine and Cellular Longevity, 2020. https://doi.org/10.1155/2020/9829176
Elma, C., Handayani, K., Azzahra, F., & Yogyakarta, I. (2024). Penetapan rendemen dan kandungan kimia ekstrak daun pepaya (Carica papaya L.) berdasarkan perbedaan konsentrasi pelarut, 20(4), 447–453.
Gusti, A. M. T., Qusti, S. Y., Alshammari, E. M., Toraih, E. A., & Fawzy, M. S. (2021). Antioxidants-related superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione-S-transferase (GST), and nitric oxide synthase (NOS) gene variants analysis in an obese population: A preliminary case-control study. Antioxidants, 10(4). https://doi.org/10.3390/antiox10040595
Kamoda, A. P. M. D., Nindatu, M., Kusadhiani, I., Astuty, E., Rahawarin, H., & Asmin, E. (2021). Uji aktivitas antioksidan alga cokelat Saragassum sp. dengan metode 1,1-difenil-2-pikrilhidrazil (DPPH). PAMERI: Pattimura Medical Review, 3(1), 60–72.
Khairunnisa, A., Amelia, A. R., & Fikriyan, F. (2023). Karakterisasi dan skrining fitokimia simplisia daun pepaya (Carica papaya L.). Pharmacine: Journal of Pharmacy, Medical and Health Science, 4(1), 1–10.
Manao, M., Karo, R. M. B., & Razoki, R. (2024). Uji aktivitas antioksidan dari fraksi etil asetat ekstrak metanol daun kerai payung (Filicium decipiens). Jambura Journal of Health Science and Research, 6(1), 306–318. https://ejurnal.ung.ac.id/index.php/jjhsr/article/view/26222
Midah, Z., Fajriansyah, F., Makmun, A., & Rasfahyana, R. (2021). Hubungan obesitas dan stres oksidatif. UMI Medical Journal, 6(1), 62–69.
Putri, M. D., Arumasi, A., & Kurniaty, N. (2020). Review artikel: Uji aktivitas antioksidan ekstrak daging buah semangka dan albedo semangka (Citrullus lanatus) dengan metode DPPH dan FRAP. Prosiding Farmasi, 10(6), 24206.
Razoki, R. (2023). Antioxidant and antibacterial activities of ethanol extract of matoa (Pometia pinnata) leaves. Journal of Pharmaceutical and Sciences, 351–357.
Rumpf, J., Burger, R., & Schulze, M. (2023). Statistical evaluation of DPPH, ABTS, FRAP, and Folin–Ciocalteu assays to assess the antioxidant capacity of lignins. International Journal of Biological Macromolecules, 233, 123470. https://doi.org/10.1016/j.ijbiomac.2023.123470
Santi, I., Abidin, Z., & Asnawi, N. (2021). Aktivitas antioksidan dari tumbuhan pepaya (Carica papaya L.). As-Syifaa: Jurnal Farmasi, 13(2), 102–107.
Sayakti, P. I., & Hidayatullah, M. (2023). Penetapan kadar fenolik total ekstrak etil asetat buah okra hijau (Abelmoschus esculentus L.). Journal of Islamic Pharmacy, 8(2), 56–61. https://doi.org/10.18860/jip.v8i2.21066
Sharma, A., Sharma, R., Sharma, M., Kumar, M., Barbhai, M. D., Lorenzo, J. M., Sharma, S., Samota, M. K., Atanassova, M., Caruso, G., Naushad, M., Radha, Chandran, D., Prakash, P., Hasan, M., Rais, N., Dey, A., Mahato, D. K., Dhumal, S., & Mekhemar, M. (2022). Carica papaya L. leaves: Deciphering its antioxidant bioactives, biological activities, innovative products, and safety aspects. Oxidative Medicine and Cellular Longevity, 2022, 1–21. https://doi.org/10.1155/2022/2451733
Sudarwati, L. P., & Fernanda, M. A. (2019). Aplikasi pemanfaatan daun pepaya sebagai biolarvasida. Gresik: Penerbit Graniti.
Warraich, U. E. A., Hussain, F., & Kayani, H. U. R. (2020). Aging – Oxidative stress, antioxidants and computational modeling. Heliyon, 6(5), e04107. https://doi.org/10.1016/j.heliyon.2020.e04107
Wulandari, R. T. (2021). Uji antioksidan ekstrak n-heksana dari kulit umbi wortel (Daucus carota L.) dengan metode DPPH (1,1-difenil-2-pikrilhidrazil). STIKES Bhakti Husada Mulia Madiun, 3–45.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2025 Jurnal Ilmu Kedokteran dan Kesehatan Indonesia
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
