Uji Aktivitas Antioksidan Ekstrak Etanol Herba Sirih Cina (Peperomia pellucida L.) dengan Metode DPPH
DOI:
https://doi.org/10.55606/jikki.v5i2.6142Keywords:
Peperomia pellucida, antioxidant, DPPH, IC₅₀Abstract
Free radicals are reactive compounds that can cause damage to cells, enzymes, lipids, and even DNA, thus triggering various degenerative diseases. Antioxidants neutralize free radicals and prevent oxidative damage. Chinese betel herb (Peperomia pellucida L.) is known to contain phenol and flavonoid secondary metabolite compounds that act as natural antioxidants. This study aims to test the antioxidant activity of ethanol extract of Chinese betel herb with DPPH (2,2-diphenyl-1-picrylhydrazyl) method. Extraction was done by maceration method using 80% ethanol solvent. Antioxidant activity was tested by reacting the extract against DPPH solution and measuring the absorbance using UV-Vis spectrophotometry. The results showed an IC₅₀ value of 32.97 ppm, which is classified as very strong antioxidant activity. These results indicate that ethanol extract of Chinese betel herb has great potential as a source of natural antioxidants.
References
Dienilah, A. (2022). Formulasi sediaan nanoemulsi ekstrak buah stroberi (Fragaria sp) sebagai bahan aktif pembuatan serum antioksidan [Skripsi, Universitas tidak disebutkan].
Handayani, R. P., Puspariki, J., & Permatasari, W. (2024). Studi anatomi sirih cina (Peperomia pellucida) segar yang tumbuh di Kabupaten Purwakarta. Journal of Holistic and Health Sciences (Jurnal Ilmu Holistik dan Kesehatan), 8(1), 25–31.
Ibroham, M. H., Jamilatun, S., & Kumalasari, I. D. (2022). A review: Potensi tumbuhan-tumbuhan di Indonesia sebagai antioksidan alami. In Prosiding Seminar Nasional Penelitian LPPM UMJ (Vol. 1).
Kartikawati, E., Hartono, K., Rahmawati, S. M., & Kusdianti, I. K. (2023). Aktivitas antibakteri ekstrak dan fraksi daun sirih cina (Peperomia pellucida L.) terhadap bakteri Propionibacterium acnes ATCC 1223. Jurnal Medika & Sains (J-MedSains), 3(1), 21–34.
Laksono, B. A., Rif’at, N. A., Arsyah, T. A., Hanifah, E. A., & Wiela, E. (2023). Evaluation of oral preparations of vitamin E as antioxidant using DPPH method (Diphenyl Picrylhydrazyl). [Artikel ilmiah, institusi tidak disebutkan].
Razoki, R. (2023). Antioxidant and antibacterial activities of ethanol extract of matoa (Pometia pinnata) leaves. Journal of Pharmaceutical and Sciences, 351–357.
Rizki, M. I., Nurlely, N., & Fadlilaturrahmah, F. (2021). Skrining fitokimia dan penetapan kadar fenol total pada ekstrak daun nangka (Artocarpus heterophyllus), cempedak (Artocarpus integer), dan tarap (Artocarpus odoratissimus) asal Desa Pengaron Kabupaten Banjar. Jurnal Insan Farmasi Indonesia, 4(1), 95–102.
Simanjuntak, E. J., & Zulham, Z. (2020). Superoksida dismutase (SOD) dan radikal bebas. Jurnal Keperawatan dan Fisioterapi (JKF), 2(2), 124–129.
Wulandari, Y. W., & Sutardi, S. S. (2021). Uji aktivitas antioksidan air mawar (rose water) dari petal bunga mawar merah (Rosa damascena Mill) menggunakan metode DPPH (Diphenyl Picril Hidrazil). Agrointek: Jurnal Teknologi Industri Pertanian, 15(3), 894–900.
Yanti, N. P. R. D., Anggreni, N. P. P. C., Pratiwi, K. A. P., Udayani, N. N. W., & Adrianta, K. A. (2023). Uji aktivitas antioksidan. [Judul jurnal tidak lengkap, mohon lengkapi untuk sitasi yang sesuai].
Yanuarty, R. (2021). Uji aktivitas antioksidan daun jeruk nipis (Citrus aurantifolia) secara spektrofotometri UV-Vis. Jurnal Farmasindo, 5(1), 53–56.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2025 Jurnal Ilmu Kedokteran dan Kesehatan Indonesia
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
