Analisis Kemometrika Kandungan Fitokimia dan Uji Anti Acne Ekstrak Etanol 70% dan Etil Asetat Daun Alpukat
(Persea Americana Mill)
DOI:
https://doi.org/10.55606/jikki.v5i1.7566Keywords:
Anti-acne, Phenolic, Flavonoid, PCA, Persea americanaAbstract
Human skin requires care to prevent and treat acne caused by Propinobacterium acnes bacteria. Researchers are exploring the use of avocado leaves as a natural therapy to treat acne. Avocado leaves contain antibacterial and anti-inflammatory compounds such as flavonoids, saponins, and alkaloids. This study aims to measure the total flavonoid and phenolic content in extracts obtained with 70% ethanol and avocado ethyl acetate and analyze their anti-acne activity. Total flavonoids were determined using a reagent consisting of 10% AlCl3 and 5% acetic acid. Total phenolic determination was carried out using the Folin-Ciocalteu reagent with gallic acid as a reference standard. Measurement of phytochemical levels used the UV-Vis spectrophotometry method. Antibacterial testing was carried out using Staphylococcus aureus bacteria with concentrations of 12%, 14%, and 16%. The positive control used clindamycin. The negative control used 10% DMSO. The disc diffusion method was used to observe the inhibition zone in this study. The results of this study will be processed using chemometrics to analyze complex chemical data. The data results showed that 70% ethanol extract produced total flavonoid levels of 8.6954% mgEK/mL and total phenolics of 15.4385% mgEAG/mL, higher than ethyl acetate extract with total flavonoid results of 3.418% mgEK/mL and total phenolics of 4.6052% mgEAG/mL. The results of antibacterial activity at concentrations of 12%, 14%, 16%, 70% ethanol extract were 10.5±0.70 mm; 12.1±0.28 mm; 14.3±0.57 mm, respectively, which had higher activity than ethyl acetate extract with results of 9.1±0.28 mm; 10.5±0.5 mm; 12.3±0.57 mm, the highest antibacterial activity at a concentration of 16%. Chemometric analysis using Principal Component Analysis (PCA) showed a strong positive correlation between phytochemical content and antibacterial activity. The highest antibacterial activity was found in the 70% ethanol extract at a concentration of 16%.
References
Alim, N., Hasan, T., Rusman, R., Jasmiadi, J., & Zulfitri, Z. (2022). Phytochemical Screening, Relationship Of Total Phenolic With Antioxidant Activity Of Ethanol And Methanol Extracts Of Kesambi (Schleichera Oleosa (Lour.) Oken) Bark. Jurnal Ilmiah Sains, 22(2), 118. Https://Doi.Org/10.35799/Jis.V22i2.40091
Amin, A. (2016). Determinasi Dan Analisis Finger Print Daun Miana (Coleus Scutellarioides Linn.) Sebagai Bahan Baku Obat Tradisional. Jf Fik Uinam, 4(2), 58–64.
Arina, Y., Shiyan, S., & Suprayetno, S. (2022). Analisis Kemometrik Ekstrak Akar Tunjuk Langit (Helminthostachys Zeylanica (L) ) Melalui Analisis Fourier Transformed Infrared Dari Berbagai Daerah Sumatera Selatan. Jurnal ’Aisyiyah Medika, 7(1), 243–258. Https://Doi.Org/10.36729/Jam.V7i1.790
Asmorowati, H. (2019). Penetapan Kadar Flavonoid Total Buah Alpukat Biasa (Persea Americana Mill.) Dan Alpukat Mentega (Persea Americana Mill.) Dengan Metode Spektrofotometri Uv-Vis. Jurnal Ilmiah Farmasi, 15(2), 51–63. Https://Doi.Org/10.20885/Jif.Vol15.Iss2.Art1
Fadel, M. N., Setyowati, E., Besan, E. J., & Rahmawati, I. (2024). Efektivitas Antidiabetes Ekstrak Etanol Bunga Telang (Clitoria Ternatea L.) Metode Induksi Aloksan. Ijf (Indonesia Jurnal Farmasi), 8(2), 60–71. Https://Doi.Org/10.26751/Ijf.V8i2.2251
Nur, S., Nursamsiar, N., Khairuddin, K., Megawati, M., & Fadri, A. (2021). Penentuan Kadar Flavonoid Dari Ekstrak Etanol Dan Etil Asetat Daun Kesambi (Schleichera Oleosa. L). Acta Pharmaceutica Indonesia, 46(2), 33–37. Https://Doi.Org/10.5614/Api.V46i2.15772
Purnamasari, A., Zelviani, S., Sahara, S., & Fuadi, N. (2022). Analisis Nilai Absorbansi Kadar Flavonoid Tanaman Herbal Menggunakan Spektrofotometer Uv-Vis. Teknosains: Media Informasi Sains Dan Teknologi, 16(1), 57–64. Https://Doi.Org/10.24252/Teknosains.V16i1.24185
Retnaningsih, A., Primadiamanti, A., & Febrianti, A. (2019). Inhibitory Test Of Purple Leaf Ethanol Extract (Graptophyllum Pictum (L.) Griff) On Staphylococcus Epidermidis Bacteria And Propionibacterium Acnes Bacteria Causes Of Acne With Discussion Methods Uji Daya Hambat Ekstrak Etanol Daun Ungu (Graptophyllum Pic. Jurnal Analis Farmasi, 4(1), 1–9.
Sam, S., Malik, A., & Handayani, S. (2016). Penetapan Kadar Fenolik Total Dari Ekstrak Etanol Bunga Rosella Berwarna Merah (Hibiscus Sabdariffa L.) Dengan Menggunakan Spektrofotometri Uv-Vis. Jurnal Fitofarmaka Indonesia, 3(2), 182–187. Https://Doi.Org/10.33096/Jffi.V3i2.220
Sy. Pakaya, M., Ain Thomas, N., Hasan, H., H. Hutuba, A., & Mbae, G. (2023). Isolasi, Karakterisasi, Dan Uji Antioksidan Fungi Endofit Dari Tanaman Batang Kunyit (Curcuma Domestica Val.,). Journal Syifa Sciences And Clinical Research, 5(2), 220–231. Https://Doi.Org/10.37311/Jsscr.V5i2.20341
Siregar, I. P. (2020). Aktivitas Anti Bakteri Mandi Celup Daun Binahong Dalam Membantu Mengurangi Jerawat Punggung. HEJ (Home Economics Journal), 4(2), 56–61.
Tivani, I., Amananti, W., Putri, A. R., & Bersama, P. H. (2021). Uji Aktivitas Antibakteri handwash EkstrakDaun Turi(Sesbania grandiflora L) TerhadapStaphylococus aureus. 7(1), 86–91.
Utami, P. R.,Indrayati, S., & Satya, W. (2022). Kombinasi Ekstrak Lidah Buaya dengan Antibiotik Ciprofloxacin dapat Menghambat Pertumbuhan Bakteri Escherichia coli. Jurnal Kesehatan Perintis (Perintis’s Health Journal), 9(1), 7–14.
Tivani, I., Amananti, W., & Rima Putri, A. (2021). Uji Aktivitas Antibakteri Handwash Ekstak Daun Turi (Sesbania Grandiflora L) Terhadap Staphylococus Aureus. Jurnal Ilmiah Manutung, 7(1), 86–91.
Yogaswara Purnomo, H., & Azzahra, F. (2021). Uji Aktivitas Antibakteri Ekstrak Etanol Daun Alpukat (Persea Americana Mill.) Terhadap Bakteri Pseudomonas Aeruginosa. Jurnal Kefarmasian Akfarindo, 7–14. Https://Doi.Org/10.37089/Jofar.Vi0.102
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